门静脉血K-ras与p53基因突变预测大肠癌肝脏微转移

K-ras and p53 Genic Mutation in Portal Vein Blood

目的研究门静脉血Ｋ一ｒａｓ、ｐ５３基因与大肠癌肝转移的关系。方法对３０例大肠癌患者的癌组织、门静脉血、外周静脉血分别检测相关基因突变，随访手术后２年内肝转移率并作统计学分析。结果门静脉血Ｋ－ｒａｓ、ｐ５３基因突变阳性率分别为２０％与３３．３％，与Ｄｕｋｅｓ分期有显著关系，与术后异时相肝转移有显著关系。结论检测门静脉血Ｋ一ｒａｓ、ｐ５３基因突变对大肠癌患者预后判断有帮助．对早期发现肝微小转移灶有意义。

To explore the relationship between K - ras and p53 genie mutation in portal vein blood and hepatic metastasis of large intestine carcinomas. Methods Mutational target genes (K - ras and p53 ) were detected in the neoplastic tissues, portal vein blood and peripheral blood of the large intestine carcinoma cases. Postoperative follow - up was conducted for two years to examine the liver metastasis. Results The positive rates for K - ras and p53 were 20% and 33. 3% respectively in the portal vein blood which were closely correlated to Duke's staging and heterochronic hepatic metastasis statistically. Conclusion Detection Detection of K - ras and p53 in the portal vein blood could help make the prognosis and early diagnosis of hepatic metastasis of large intestine carcinomas.