浙江枝吻纽虫(Dendrorhynchus zhejiangensis)凝溶胶蛋白基因的原核表达产物抗肿瘤活性的研究

THE ANTI-TUMOR ACTIVITIES OF PROKARYOTIC RECOMBINANT EXPRESSION PRODUCT OF GELSOLIN GENE FROM DENDRORHYNCHUS ZHEJIANGENSIS

凝溶胶蛋白(Gelsolin)作为一种重要的肌动蛋白调节蛋白,在机体的多种生理和病理活动中发挥重要作用。本文采用细胞MTT染色法,Hoechst33342/PⅠ荧光染色和流式细胞仪技术,对浙江枝吻纽虫(Dendrorhynchus zhejiangensis)的gelsolin基因的原核表达产物(r-gelsolin)进行了体外抗肿瘤活性的研究。结果表明,30μg/ml的r-gelsolin作用于胃低分化粘液腺癌MGC803细胞24h后,细胞的增殖抑制率达到最高,约为34.53%。荧光染色显示,r-gelsolin使MGC803细胞的正常形态发生改变,细胞核出现核固缩,破裂,产生凋亡小体等现象。进一步通过流式细胞仪证实,r-gelsolin可以阻滞MGC803细胞于S、G2期,影响细胞分裂的进程,并促使细胞发生凋亡。

Gelsolin is an important actin binding protein and involved in many cellular processes. In this work, anti-tumor activities of the prokaryotic recombinant expression product of gelsolin gene from Dendrorhynchus zhejiangen- sis were assessed employing the MTT assay, Hoechst33342/PI staining and a flow cytometric analysis. The results showed that the growth inhibition rate of MGC803 cells was reached to 34.53% when the cells were treated by 30~tg/ml r-gelsolin for 24h. Effects of r-gelsolin on the morphology of MGC803 cells were observed by Hoechst33342/PI staining. The cell nuclei showed pyknosis, rupture, and apoptotic bodies appeared. Furthermore, cell cycle and apoptosis were detected by flow cytometer, compared with the control group, the results showed that r-gelsolin could arrest the cells in S/G2 phase, inhibit the proliferation of cells, and induce their apoptosis.