大菱鲆(Scophthalmus maximus)耐高温品系选育及耐温性能评估

THE SELECTIVE BREEDING OF THERMAL TOLERANCE FAMILY AND APPRAISAL OF PERFORMANCE IN TURBOT SCOPHTHALMUS MAXIMUS

采用家系选育方法,进行了大菱鲆耐高温性状选育研究,对两代家系耐温性能进行测定,并利用bCOX回归分析了各家系的耐温性优势比。结果表明:各家系耐温性差异达到了显著水平(P<0.05),并且耐高温能力的优势比也存在显著差异,这反映出在大菱鲆耐高温选育中家系选择的高效性;经过一代选育,获得4个耐高温家系,并据此构建选育二代家系。耐温性能评估,选育二代比一代有显著提高,在27℃高温条件下,选育一代的平均死亡率为49%,选育二代的平均死亡率为10.26%;28℃条件下,选育二代耐高温子代的平均死亡率为32.83%,对照组的平均死亡率为88.9%;经过筛选后,选育二代的耐温性比选育一代提高了1—2℃左右。

In order to carry out the breeding program which about thermal tolerance traits of turbot Scophthalmus maximus L., a large-scale family construction was carry out, and detected the tolerance performance of two generation, and calculate the odd ratio of tolerance by bCOX regression analysis. The results demonstrated that the tolerance and it odd ratio of every family have been at a significance different level （P〈0.05）, it reflected that the high performance of family breeding method. After the first filial generation breeding, we get four tolerance family, and constructed the second filial generation, to evaluate the thermal tolerance, the result suggested that mortality rate of F2 family just 10.25%, and FI fam- ily was 49%, the F2 family tolerance was higher than F1, when they in the 27℃condition. Then in the 28℃ condition, F2 family the mortality rate just 32.83%, and the mortality of control group was 88.9%. According to these analysis, we judged that the tolerance of F2 family have increased 1--2℃.