基于SSR标记的魁蚶(Scapharcabroughtonii)不同群体遗传结构的分析

GENETIC VARIATION IN DIFFERENT POPULATIONS OF SCAPHARCA BROUGHTONII SCHRENCK INFERRED FROM MICROSATELLITE DATA

运用6对微卫星分子标记分析了山东蓬莱(SP)、山东黄岛(SH)、江苏前三岛(JQ)、韩国统营(Korea)等魁蚶4个地理群体的遗传结构。结果表明,4个魁蚶群体在6个位点的等位基因数(a)为11.3333—14.3333,平均有效等位基因数(ae)为6.2977—9.5873;平均杂合度观察值(Ho)为0.4083—0.5641,平均杂合度期望值(He)0.7849—0.8753,各位点平均多态信息含量(PIC)值为0.7703—0.9102,遗传多样性非常丰富。聚类分析结果表明黄岛群体与前三岛群体聚在一起,蓬莱群体与统营群体聚在一起最后聚类。本研究结果可为今后魁蚶种质资源保护和遗传育种提供遗传学资料。

The microsatellite DNA technique was applied to estimate genetic structure of the four different geographi- cal populations of Scapharca broughtonii. Using six microsatellite loci, genetic parameters of the four populations were calculated： heterozygosity, polymorphism information content （PIC）, effective allele number, genetic distance and so on. The result indicated that the four populations were in good condition. FsT values showed significant genetic differentiation among the four populations except that between the populations of JQ and SH. The Neighbour-joining tree topology constructed on the genetic distances among populations showed a clear division of them. The information on the genetic distances and diversity in the four geographical populations S. broughtonii in this study is useful for setting up suitable guidelines for founding and maintaining of cultured stocks, and for future genetic improvement by selective breeding.