酸性铬兰K分光光度法测定血红蛋白

Spectrophotometric determination of hemoglobin with acid chrome blue K

在pH 3.2的Clark-Lub’s缓冲溶液中,酸性铬兰K与血红蛋白发生反应,使体系吸光度增大,在375 nm处的吸光度增加值与血红蛋白的加入量成正比,据此建立了测定血红蛋白的分光光度法。探讨了测定血红蛋白的最佳条件和干扰情况,方法的线性范围0～112μg/mL,表观摩尔吸光系数ε375为4.7×105L.mol-.1cm-1,方法已用于动物血液和人尿液中血红蛋白含量的测定。

In Clark-Lub＇ s buffer solution of pH 3.2, acid chrome blue K reacted with hemoglobin to form a complex, the absorbance could be enhanced, and the enhanced absorbance value at the wavelength of 375 nm was proportional to the concentration of hemoglobin. Based on this, a new spectrophotometric method was developed for the determination of hemoglobin. The optimum conditions and effects of foreign ions were investigated. The results indicated that hemoglobin concentration demonstrated good linear correlation in the range of 0 ~ 112 μg/mL, and the apparent molar absorptivity ε375 was 4.7 ~ 10s L. mol-l· cm This method has been applied to the determination of hemoglobin concentration in animal blood and human urine samples with satisfactory results