分子印迹固相微萃取测定中成药的鸟苷含量

Determination of guanosine in chinese traditional patent medicine with sold phase microextraction by molecularly imprinted monolith

用自制的分子印迹整体柱作固相微萃取介质,结合HPLC-UV装置,建立了中成药鸟苷成分的萃取检测方法。方法的线性范围为0.01～5.30μmol/L,检测限(LOD)为2.5 nmol/L(S/N=3),定量限(LOQ)9.0 nmol/L(S/N=10),样品加标回收率在94%～104%之间。方法已用于刺五加片、板蓝根冲剂及清热口服液等中成药鸟苷成分的检测。

Coupled to HPLC-UV, a novel method for the determination of guanosine in chinese traditional patent medicine was constructed with molecularly imprinted monolith used as media of solid phase microextraction. Under the selected conditions, the linear range of determination was from 0.01 p, mol/L to 5.30 Ixmol/L, the limit of detection （LOD） was 2.5 nmol/L （ S/N = 3 ） and limit of quantitation was 9.0 nmoL/L （ S/N = 10 ）. Recoveries for spiked standards were between 94% and 104% with averages of 99% ＋ 5%. This new method was successfully applied in the analysis of several kinds of Chinese traditional patent medicine.