盐生植物小花碱茅外整流K^+通道SKOR基因片段的克隆及序列分析

Cloning and sequence analysis of outward-rectifying potassium channel SKOR gene fragment from halophyte Puccinellia tenuiflora

为研究小花碱茅(Puccinellia tenuiflora)K+/Na+选择性运输的分子机制,根据已知的外整流K+通道高度保守区设计一对简并性引物,以小花碱茅根总RNA为模板,采用RT-PCR方法克隆SKOR基因片段。序列分析结果表明,该基因片段长度为555bp,编码185个氨基酸;该序列与其他已报道的高等植物SKOR核苷酸序列的同源性在66%以上,氨基酸序列同源性在55%以上。

To investigate molecular mechanism of K＋/Na＋ slective transport in Pucciinellia tenuiflora, degenerate primers were designed based on the conserved sequences of the outward-rectifying potassium channel from other plants. Total RNA was extracted from the roots of P. tenuiflora. SKOR gene fragment was obtained by reverse transcription polymerase chain reaction （RT-PCR）, which could provide basis for cloning full-length SKOR gene,expression regulation and RNAi. The sequencing result showed that the SKOR gene fragment from P. tenuiflora contained about 555 bp, encoding 185 amino acids. Homology comparison with SKOR gene sequences of other higher plants showed that it shared over 66% nucleotide sequence homology and 55% amino acid sequence homology.