海洋细菌LP621右旋糖苷酶分批发酵动力学研究

Kinetics analysis for dextranase of Pseudoalteromonas tetraodonis LP621 by liquid fermentation

对海洋细菌Pseudoalteromonas tetraodonis LP621右旋糖苷酶进行了50L发酵罐分批发酵的代谢特性的研究。结果表明LP621右旋糖苷酶合成和菌体生长呈部分生长偶联型。据分批发酵试验结果采用Logistic方程、Luedeking-Piret方程建立了描述菌株生长、产酶以及葡萄糖底物消耗分批发酵动力学模型。动力学模型计算值结果与实验值拟合良好,较好反映了菌株右旋糖苷酶分批发酵过程的动力学特征。

The kinetics for dextranase of marine bacteria Pseudoalteromonas tetraodonis LP621 by liquid fermentation was studied. The results showed that the dextranase production was partially associated with the growth of LP621. Based on the result of batch fermentation, the kinetic models for cell growth, dextranase formation, sugar consumption were set up using Logistic equation and Luedeking-Piret equation. The calculated results of models well matched the experimental data. The model equations could well reflect the dextranase fermentation process and kinetic characteristic.