响应面法优化超声波辅助提取胡桃楸种仁壳总黄酮工艺

Optimization of ultrasonic-assisted extraction of total flavonoids from seed shell of Juglans mandshurica by response surface methodology

为确定胡桃楸种仁壳总黄酮超声波辅助提取的最佳工艺条件,以总黄酮得率和总还原力为指标,进行单因素实验,并在此基础上,选取超声波功率、超声波时间、料液比为影响因素,采用响应面法进行优化并得到回归模型。结果表明:最优工艺条件为超声波功率250W,超声波时间31min,料液比1:31(g/mL)。回归模型预测的黄酮得率理论值为6.70mg/g,经验证实验,RSD为1.47%,该回归方程与实际情况拟合较好。

To optimize extraction yield of total flavonoids from seed shell of Juglans mandshurica under ultrasonic assistance,single factor test were carried out at extraction yield of total flavonoids and total reducing power as index.Based on this, power of ultrasonic-assisted extraction, time of ultrasonic-assisted extraction and solid-toliquid ratio were selected as variables.And a mathematical model was obtained according to response surface methodology. Results showed that the following optimum parameters, power of ultrasonic-assisted extraction, 250W; time of ultrasonic- assisted extraction,3 1 min ; and solid- to- liquid ratio, 1 ：31 （g/mL） .The predicted value of extraction yield of total flavonoids was 6.70mg/g.After confirmatory test,the relative standard deviation was 1.47%. The regression equation was fitting better with actual situation.