天然苹果香精成分含量测定方法的优化

Method optimization of content determination of the main components in natural apple essence

【目的】建立了天然苹果香精中14种主要成分顶空固相微萃取法(HS-SPME)及气相色谱及质谱联用(GC-MS)含量测定的方法。【方法】通过单因素及响应曲面法实验确定HS-SPME最佳条件;通过内标标准法建立标准曲线,并测定香精成分。【结果】最佳的HS-SPME条件是85μmPA萃取头、香精体积2mL、40℃萃取温度、NaCl浓度0.4g/mL、萃取时间30min。标准曲线测定苹果香精含量中精密度实验RSD1.20%～3.60%,重复性实验RSD3.17%～8.86%,标准品回收率93.44%～107.42%。【结论】HS-SPME/GC-MS结合内标标准法测定有较好的准确性、精密度、重复性,能够有效测定苹果香精中成分含量。

[ Objective] Established the method to determine the contents of 14 main compounds in natural apple essence using Head Space-Solid Phase Microextraction,and gas chromatography mass spectrometry. [ Method ] The optimal HS-SPME condition was obtained through single factor and surface response design experiments. Standard curve was established by internal standard method to determine compounds＇ contents. [ Result]The optimal conditions for HS-SPME were 85μm PA fiber,2mL natural apple essence,40 ℃ water bath,0.4g/mL NaCI, 30min of extracting time. Results of content determination of compounds experiments：the precision experiment results RSD was 1.20% -3.60%;the repeatability experiment results RSD was 3.17% -8.86%; the recovery of chemical standards was 93.44%-107.42%. [ Conclusion ] The method of HS-SPME/GC-MS coupled with internal standard curve provides high accuracy, precision, repeatability, was effective for content determination for compounds in apple essence.