甲状腺乳头状癌PI3K/Akt信号转导通路mRNA和蛋白表达及其生物学意义的探讨

Expression and biologically significant of PI3K/Akt signal transduction pathway in papillary thyroid carcinoma

目的:探讨甲状腺乳头状癌(PTC)中PI3K mRNA、Akt mRNA、PI3K和Akt的表达与意义。方法:采用逆转录聚合酶链反应(RT-PCR)测定组织中PI3KmRNA和Akt mRNA的表达;应用免疫组化法测定组织中PI3K和Akt蛋白的表达。结果:PTC中PI3KmRNA表达量(90.3±5.06)和Akt mRNA的表达量(78.13±5.5)高于癌旁正常甲状腺组织(NAT)表达量37.74±10.63和14.82±5.63,P<0.05。免疫组化结果显示,甲状腺乳头状癌PI3K蛋白表达阳性率73.33%、Akt蛋白表达阳性率(83.33%)明显高于NAT组织蛋白表达阳性率33.33%和16.67%,P<0.05。PTC中PI3K和Akt蛋白表达与包膜浸润及淋巴结转移相关,P<0.05。结论:PTC中存在PI3K/Akt信号传导通路的异常激活,信号传导途径的激活水平与PTC浸润和局部转移有关。

OBJECTIVE： To investigate the expressions of PI3K mRNA, Akt mRNA PI3K and Akt in papillary thyroid carcinoma（PTC） and non carcinoma thyroid （NAT） tissue, and explore the pathogenesis of PTC. METHODS： RT-PCR method was applied to detect the expressions of PI3K mRNA and Akt mRNA. Immunohistochemistry methods were used to test the pro tein expression of PI3K and AkE RESULTS： The expressions of PI3K mRNA and Akt mRNA in PTC were significantly higher than those innoncarcinomathyroid（NAT）（（90.3±5.06）, （78. 13±5.5）vs （37. 74±10. 63）, （14.82±5. 63）,both P〈 0. 05）. The results of immunohistochemistry discovered that expressions positive rate of PI3K and Akt in PTC were higher than those in NAT （73.33^, 83.33^ vs 33.33^, 16.67% ,both P〈0.05）. The contents of PI3K and Akt protein were related to the lymph node metastasis and the extracapsular spread of PTC （P〈0. 05）. CONCLUSIONS： PI3K/Akt signal transduction pathway is activated abnormally in PTC. The activation level of PI3K/Akt signal transduction pathway is ralated to the malignan- cy degree and lymphatic metastasis in the PTC.