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钙离子对重组创伤弧菌溶细胞素诱导THP-1细胞损伤的影响机制 被引量：1

The role of calcium in the rVvhA induced THP-I cells apoptosis
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摘要 目的研究重组创伤弧菌溶细胞素（rVvhA）诱导人单核细胞白血病细胞（THP-1）的凋亡机制及其Ca2＋的变化。方法采用CCK-8法、激光共聚焦显微镜结合Fluo3／AM法、流式细胞术结合AnnexinV-PI标记等检测rVvhA对THP-1细胞的影响，并观察胞内Ca2＋浓度变化。结果rVvhA可诱导THP-1细胞发生凋亡并引起细胞内ca2＋浓度升高，细胞内钙离子螯合剂BAPTA—AM处理组胞内钙离子升高幅度远高于细胞外钙离子螯合剂EGTA处理组。结论rVvhA具有诱导THP-1细胞凋亡的生物学活性，并能引起细胞内ca2＋浓度升高，升高的Ca2＋主要源于胞外钙离子内流。 Objective To study the role of Vibrio vulnifwus cytolysin（rVvhA） induced THP-1 ap- optosis and calcium influx. Methods CCK-8 cell proliferation kit, Fluo3/AM staining and AnnexinV/PI staining were performed to identify the apoptosis and calcium influx induced by rVvhA in THP-1 ceils. Results rVvhA could induce THP-1 apoptosis and up-regulate the cellular calcium concentration. BAPTA- AM could enhance the calcium influx induced by rVvhA in THP-1. Conclusion rVvhA had cytotoxic to THP-1 cells bv inducing apoptosis and triggering extracellular calcium influx.
作者 王永明 郭秋 史玢 谢旦立 楼永良
机构地区 浙江省诸暨市人民医院检验科 温州医学院检验医学院 检验医学省部共建教育部重点实验室
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2012年第6期512-518,共7页 Chinese Journal of Microbiology and Immunology
基金 浙江省自然科学基金（Y2090468）
关键词 创伤弧菌 溶细胞素 钙离子 人单核细胞白血病细胞 Vibrio vulnificus Cytolysin Calcium THP-1
分类号 R378 [医药卫生—病原生物学]
  • 相关文献

参考文献14

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二级参考文献37

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共引文献19

同被引文献14

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引证文献1

中华微生物学和免疫学杂志
2012年 第6期

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