罗格列酮对HaCaT细胞增殖及β-连环蛋白、细胞周期蛋白D1表达的影响

Effects of rosiglitazone on the proliferation of and expressions of β-catenin and cyclin D1 in HaCaT cells

目的探讨罗格列酮对体外培养的角质形成细胞株HaCaT细胞体外增殖的影响。方法用不同浓度的罗格列酮处理HaCaT细胞，四甲基偶氮唑蓝（MTT）法检测HaCaT细胞增殖活性；蛋白免疫印迹法测定HaCaT细胞B-连环蛋白、细胞周期蛋白D1表达水平。结果不同浓度的罗格列酮对HaCaT细胞体外增殖均具有抑制作用，药物作用48h后对HaCaT细胞体外增殖的抑制率分别为18．9％、23．7％、35．1％、44．6％，与溶媒组相比，差异有统计学意义（P〈0．05）。罗格列酮处理HaCaT细胞后，p-连环蛋白、细胞周期蛋白D1表达明显下调。结论罗格列酮抑制HaCaT细胞的体外增殖的机制可能与B-连环蛋白、细胞周期蛋白D1的表达下调有关。

Objective To estimate the effects of rosiglitazone on cultured HaCaT human keratinocytes and their possible mechanism. Methods HaCaT ceils were cultured and treated with different concentrations （10, 20, 40, 80 μmol/L） of rosiglitazone or solvent for 24, 48, 72 and 96 hours, respectively. Cell proliferation was detected with methyl thiazolyl tetrazolium （MTT） assay. Western blot was performed to measure the protein expression of β- catenin and cyelin D1. Results Compared with the solvent-treated cells, the proliferation of HaCaT cells was significantly inhibited by 18.9%, 23.7%, 35.1% and 44.6% （all P 〈 0.05） after treatment with rosightazone of 10, 20, 40 and 80 μmol/L, respectively, for 48 hours. The expressions of β-catenin and cyclin D1 were significantly lower in rosiglitazone-treated HaCaT cells than in solvent-treated cells （all P 〈 0.05）. Conclusion Rosiglitazone could inhibit the proliferation of HaCaT cells, likely by downregulating the expressions of β-catenin and cyclin D1.