大疱性类天疱疮患者骨桥蛋白和白介素18的检测

Detection of osteopontin and interleukin-18 in patients with bullous pemphigoid

目的探讨骨桥蛋白（OPN）、白介素（IL）18在大疱性类天疱疮中的作用及其与临床和实验室指标的相关性。方法酶联免疫吸附法（ELISA）对30例大疱性类天疱疮患者和30例健康对照血清中OPN、IL-18的浓度进行测定。结果BP组血清OPN的表达水平高于健康对照组（P〈0．01），且与疾病活动性、部分化验室指标有相关性。BP患者有心脑血管疾病者，血清IL-18的表达明显高于无心脑血管疾病者（P〈0．01）。伴有糖尿病、肿瘤或肝肾损害者OPN与IL-18的表达均明显高于无糖尿病、肿瘤或肝肾损害者（P〈0．05或P〈0．01）。结论OPN可能与BP病情活动性呈正相关，IL-18可能与BP合并症的发生有关。

Objective To detect the expressions of osteopontin （OPN） and inerleukin-18 in patients with bullous pemphigoid （BP）, and to analyze their relationship with clinical and laboratory indices. Methods Enzyme linked immunosorbent assay （ELISA） was performed to quantify the serum levels of osteopontin （OPN） and inerleukin （IL）-18 in 30 patients with BP and 30 health controls. Results The serum level of OPN was statistically higher in the patients than in the healthy controls （（8.29 ± 2.76） vs. （3.88 ±1.41 ） ng/ml, P 〈 0.01 ）, and was positively correlated with the severity of BP （r = 0.658, P 〈 0.01 ） and with some laboratory indices in the patients. Increased serum IL-18 level was observed in patients complicated by cardiovascular diseases compared with those without cardiovascular diseases （（37.49 ± 6.43） vs. （31.10± 5.40） pg/ml, P 〈 0.01）. Moreover, the BP patients with diabetes, tumor, hepatic and renal impairment displayed an enhanced level of serum OPN and IL-18 than those without （all P 〈 0.05）. Conclusions OPN may be positively correlated with the severity of BP, while IL-18 may be involved in the development of complications of BP.