全互穿半乳糖基水凝胶的制备与细胞活性研究

Preparation and cell viability of hydrogels based on galactosylated acrylate with full interpenetrating polymer network structure

采用两步法,分别以无机纳米粘土硅酸镁锂（LMSH）和亚甲基双丙烯酰胺（MBA）作为异丙基丙烯酰胺（NIPAm）和氨基半乳糖丙烯酸衍生物（GAC）的物理和化学交联剂,制备得到全互穿的P（NIPAm/LMSH）/P（GAC/MBA）水凝胶;采用红外光谱、扫描电镜和称重法研究了全互穿水凝胶的化学结构、内部孔洞、（消）溶胀和温度脉冲响应性行为,并探索了凝胶表面的细胞活性.结果表明：全互穿水凝胶孔洞数量多,尺寸为20~30μm;10 min内溶胀度增加1.4倍,失水率超过60%,并表现出良好的温度脉冲响应性,且随脉冲次数的增加,溶胀度逐渐下降;鼠成纤细胞（L929）培养结果表明2~8 d内水凝胶表面细胞数量逐渐增加,但低于聚苯乙烯组织培养板（TCPS）上的细胞数量.

Adopting two-step method,the P（NIPAm/LMSH）/P（GAC/MBA） hydrogel with full interpenetrating polymer network is prepared by using inorganic nanoclay lithium magnesium silicate hydrate（LMSH） as physical cross-linker and using N-methylenebisacrylamide（MBA） as chemical cross-linker of N-isopropylacrylamide（NIPAm） and galactosylated acrylate.The chemical structure,interior pore shape,（de）swelling behavior and stimuli-responsibility are investigated by Fourier transform infrared spectroscopy（FTIR）,scanning electron microscope（SEM） and weight method.The cell viability on the surface of resulting hydrogel is also explored.The results show that the P（NIPAm/LMSH）/P（GAC/MBA） hydrogel presents numerous pore and larger pore ranged from 20 to 30 μm.Within 10 min,the swelling ratios increase 1.4 times and water retention decreases over 60%.The hydrogel also shows excellent impulse responsibility,and with increasing impulse cycle times,the swelling ratio decreases gradually.In the case of L929 cell culture on the surface of hydrogel,cell number increases in 2-8 d,while lower than that of on the surface of tissue culture polystyrene（TCPS）.