微波消解-ICP-AES法测定葡萄皮渣中生命元素

Determination of Biological Elements in Grape Pomace by Microwave Digestion and ICP-AES

采用微波消解技术处理样品,建立了ICP-AES法测定酿酒葡萄赤霞珠、霞多丽和野生山葡萄、毛葡萄皮渣中钾、钠、钙、镁、铬、铜、铁、锰、镍、硒、锌、钴、钒、锡、钼等15种生命元素的方法。对微波消解程序(包括消解液体系、用量等)和ICP仪器操作参数进行了优化选择,并对分析方法进行了质量评价。结果表明:葡萄皮渣中含有种类丰富的生命元素,其中K、Na、Ca、Mg、Cu、Fe、Mn、Zn的含量较高,Cr、Ni、Se、Co、V、Sn及Mo的含量也不容忽视。在优化条件下,该方法的RSD为0.56%～3.21%(n=10),平均加标回收率为95.1%～110.2%(n=6)。该方法精密度高、重现性好,完全满足葡萄皮渣中多种生命元素的测定的要求。结合前人研究成果,揭示了葡萄皮渣具有较高的营养和保健价值。

An Inductively Coupled contents of 15 biological elements pomace after microwave-assisted Plasma atomic emission spectrometry （ICP-AES） for determination of the （K, Na, Ca, Mg, Cu, Fe, Mn, Zn, Cr, Ni, Se, Co, V, Sn and Mo） in grape digestion of the sample had been established. The microwave-assisted digestion procedure （including digestion system） and the techniques for operating ICP instrument were optimized, and the analytical method was validated. The results showed that the biological element species in grape pomace are abundant, among which the contents of K, Na, Ca, Mg, Cu, Fe, Mn and Zn are rather high and others are not ignored. The relative standard deviations were from 0.56 % to 3.21% （n=10）, and the average recoveries were from 95.1% to 110.2 % （n=6） under the optimized conditions. Repeated validations indicated that this method was precise, sensitive and reproducible for the determination of biological elements in various grape pomaces. The comprehensive research results of the authors and previous researchers show that grape pomace has high nutrition and health protection value.