尘肺病患者外周血中SDC-2基因的表达变化

Expression change of syndecan-2 gene in peripheral blood from patients with pneumoconiosis

目的:测定尘肺病患者外周血中SDC-2基因的表达水平,探讨检测该指标作为尘肺病相关生物标志物的可行性。方法:分别用酶联免疫吸附试验(ELISA)和实时定量RT-PCR方法,对77例尘肺病患者和162例健康体检者外周血中SDC-2基因的表达变化进行测定。结果:尘肺病患者血清SDC-2的浓度为42.37±19.32 pg/ml,健康对照组为37.08±6.24 pg/ml,两组之间的差异没有统计学意义(F=3.278,P=0.074);矽肺患者血清SDC-2的浓度水平高于煤工尘肺患者,但两者未有统计学差异(F=0.803,P=0.377);不同影像分期尘肺病患者血清中SDC-2的浓度水平差异无统计学意义(P>0.05);尘肺病患者肺功能损害程度与血清SDC-2浓度没有相关性(r=-0.159,P=0.361);SDC-2基因转录水平的变化趋势与其血清浓度的变化趋势基本一致。结论:SDC-2基因的表达变化作为尘肺病相关血液学生物标志物可能是不可行的。

Objective：To detect the expression change of syndecan -2 gene in peripheral blood from the patients with pneumoconiosis, and explore the feasibility of the index as a potential biomarker of pneumoconiosis. Methods： Human peripheral blood samples of 77 patients with pneumoconiosis and 162 healthy subjects were collected, and the changes of SDC - 2 gene expression were measured by enzyme - linked immunosorbent assay （ELISA） and real -time RT- PCR. Results： The SDC -2 concentrations in serum of the patients with pneumoconiosis were higher than those of the healthy subjects （42.37 ± 19.32 pg/ml vs. 37.08±6.24 pg/ml）, but the difference was not obvious （ F = 3. 278, P = 0. 074）. The serum SDC - 2 concentrations of patients with silicosis were higher than those of coal workers with pneumoconiosis, but there was no statistical difference （ F = 0. 803, P = 0. 377 ）. The serum SDC -2 concentrations had no significant difference among different stages of pneumoconiosis （P 〉 0.05 ）. The in- creased SDC - 2 levels were not associated with the degree of respiratory impairment （ r = - 0. 159, P = 0. 361 ）. Change trend of SDC - 2 gene in transcription level was essentially consistent with the change in serum concentration. Conclusion： The expression change of SDC - 2 gene as a hematological biomarker of patients with pneumoconiosis was not feasible.