江苏省副溶血性弧菌同源性研究

Homology analysis of Vibrio parahaemolyticus in Jiangsu

目的:了解江苏省副溶血性弧菌的指纹图谱的同源性。方法:对2009年-2010年江苏省食源性疾病中分离的、经API生化鉴定的79株副溶血性弧菌进行毒力基因(tdh、trh)测定、血清学分型和脉冲场电泳(PFGE)分型。结果:tdh阳性率82.3%(65/79),trh阳性率为3.4%(3/79);病人株血清型以O3:K6为主(44/75),食品株以O1:KUT为主(3/4);脉冲场凝胶电泳(PFGE)分型显示优势群A群-B群间相似度大于60%。结论:江苏省不同地区、不同时间的副溶血性弧菌具有高度同源性,该地区副溶血性弧菌引起的食源性疾病可能具有相同的污染源。

Objective：To understand the homology traits of Vibrio parahaemolyticus in Jiangsu.Methods：79 Vibrio parahaemolyticus strains were collected from the samples of food poison incidents in Jiangsu from 2009 to 2010.Biochemical identification,tdh and trh gene detection,serological typing and pulse-field gel electrophoresis（PFGE） were applied to the strains.Results：The positive rates of tdh and trh were 82.3% and 3.4% respectively.The main serological type of clinical strains was O3：K6,while that of food strains was O1：KUT.PFGE results showed that the similarity of group A to B was more than 60%.Conclusion：Strains of different regions and different time in Jiangsu have high homology,and food poison incidents occurred in this area may have the same pollution source.