2010年-2011年曲靖市手足口病病原学监测结果分析

Analysis of etiological surveillance of hand,foot and mouth disease during 2010-2011 in Qujing

目的:了解2010年-2011年曲靖市手足口病的病原体型别及分布特征,为手足病的防控提供病原学依据。方法:采集本市各医疗机构临床诊断为手足口病患者的粪便、咽拭子、疱疹液标本,用Real-timeRT-PCR法进行肠道病毒核酸检测和型别鉴定。结果:2010年5月-2011年12月共收集各类标本284份,检出肠道病毒核酸235份,总阳性率82.75%,其中EV71检出116份,占49.36%,Cox A16检出119份,占50.64%;发病年龄主要集中在5岁以下儿童,占92.96%;粪便中肠道病毒检出率(90.67%)最高,其次为疱疹液(88.89%)。结论:2010年引起曲靖市手足口病的病原体主要是Cox A16型,2011年引起曲靖市手足口病的病原体主要是EV71型,5岁以下儿童是肠道病毒感染的高危人群,EV71是引起重症病例的主要病原体。

Objective：To analyze the etiological characteristics of hand, foot and mouth disease（HFMD） in Qujing from 2010 to 2011 ,and provide basis for further control and prevention. Methods： Feces, throat -swab specimens and fluid from blisters were collected from patients with HFMD in different medical institutions, and the enterovirus nuclear acid detection and typing identification were done by real - time RT - PCR. Results： 235 specimens were tested positive for enterovirus from 284 specimens, the total positive rate was 82.75% from May 2010 to Dee. 2011. In all cases, 116 samples（49.36% ） were EV71 type and 119 samples（50.64% ） were CoxA16. Most cases （92.96%） were children under 5 years old. Enterovirus positive rate （90.67%） in feces was higher than that in fluid from blisters which was 88.89%. Conclusion： The major causative pathogens for HFMD in Qujing were CoxA16 in 2010 and EV71 in 2011. Children under 5 years old were high risk population, EVT1 was the predominant type which led to serious conditions.