HPLC法测定消瘤丸中姜黄素的含量

Determination of Curcumin in Xiaoliu Pills by HPLC

目的:建立消瘤丸中姜黄素含量的高效液相色谱检测法。方法:采用Hypersil ODS-2C_(18)(200 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.4%冰醋酸(45:55),流速1.0 ml·min^(-1),检测波长420 nm。结果:姜黄素在10～100μg·ml^(-1)浓度范围内,含量与峰面积呈良好的线性关系(r=0.999 2),平均加样回收率为96.6%,RSD为1.35%(n=5)。结论:该方法简便,准确,灵敏度高,重复性好,可用于消瘤丸中姜黄素的含量测定。

Objective： To establish a method for the determination of curcumin in Xiaoliu pills by HPLC. Method ： The Hypersil ODS-2 C18 column （200 mm ×4.6 mm, 5 μm） was used, the mobile phase consisted of acetonitrile-0.4% glacial acetic acid solution （ 45： 55 ） with a flow rate of 1.0 ml·min - 1. The detection wavelength was 420 nm. Result： The calibration curve showed a good line- arity over the range of 10 - 100μg · ml -1（ r = 0. 999 2）. The average recovery was 96.6% （RSD = 1.35% , n = 5 ）. Conclusion： The method is simple and accurate with high sensitivity and good repeatability. It can be used in the determination of curcumin in Xiaoliu pills.