HPLC法同时测定复方槐花口服液中柚皮苷和黄芩苷的含量

Determination of Baicalin and Naringin in Compound Huaihua Oral Liquids by HPLC

目的:建立HPLC法同时测定复方槐花口服液中柚皮苷、黄芩苷的含量。方法:色谱柱:Agilent ZORBAX SB-C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液(23∶77);检测波长:280 nm;流速:1.0 ml.min-1;柱温:30℃。结果:柚皮苷和黄芩苷分别在0.194～1.742μg.ml-1(r=0.999 5,n=5)和0.419～3.773μg.ml-1(r=0.999 8,n=5)范围内与峰面积呈良好线性关系,平均加样回收率为100.06%,RSD为1.10%(n=6)和99.27%,RSD为1.43%(n=6)。结论:HPLC法操作简便,结果准确,可用于复方槐花口服液的质量控制。

Objective： To develop an HPLC method for the determination of naringin and baicalin in compound Huaihua oral liquids. Method： The determination was carried out on an Agilent ZORBAX SB- Cls （250 mm × 4.6 mm,5 μm） column with acetonitrile- 0.1% phosphoric acid （23： 77） as the mobile phase. The flow rate was 1.0 ml min-1 and the UV detection wavelength was set at 280 nm with the column temperature of 30℃. Result： The linearity of naringin and baicalin was within the range of 0. 194-1. 742μg m- 1 （ r = 0. 999 5, n = 5 ） and 0.419-3. 773 μg ml -1 （ r = 0. 999 8, n = 5 ）, respectively. The average recovery of baicalin and naringin was 100.06% and 99.27% with RSD of 1.10% （ n = 6） and 1.43% （ n = 6）, respectively. Conclusion： The method is simple, accurate and specific, and can be used in the quality control of compound Huaihua oral liquids.