氧化应激在低压缺氧性肺动脉高压形成中的作用

The role of oxidative stress in the hypoxic pulmonary hypertension

目的观察低氧对血清中活性氧(ROS)水平的影响,探讨氧化应激在低压缺氧性肺动脉高压形成中的作用。方法将24只Wistar大鼠随机分为平原对照组(C组),缺氧组(H组),N-乙酰半胱氨酸组(N组),缺氧+N-乙酰半胱氨酸组(H+N组)。建立低压缺氧模型21 d后,测定其右心室收缩压(RVSP)和平均肺动脉压(mPAP);HE染色观察各组大鼠的肺动脉壁厚度;比色法测定血清中ROS的含量。结果与C组相比,H组RVSP、mPAP明显升高(均P<0.05),HE染色示H组肺动脉壁增厚,且血清中ROS水平明显升高(P<0.05);给予ROS清除剂NAC后,RVSP及mPAP降低,肺血管重塑减弱。结论低氧时氧化应激参与了低压缺氧性肺动脉高压的形成。

Objective To observe the effect of hypoxia on reactive oxygen species（ROS） levels in the serum and explore the role of oxidative stress in the hypoxic pulmonary hypertension. Methods Twenty four wistar rats were randomly divided into plain control group（C group）,hypoxia group（H group）,NAC group（N group） and hypoxia＋NAC group（H＋N group）.After the hypobaric hypoxia model was established for 21d,right ventricular systolic pressure（RVSP）and mean pulmonary artery pressure（mPAP） were measured,each group of pulmonary artery wall thickness were stained with HE method.The content of ROS in serum was determined by colorimetry. Results Compared with C group,RVSP and mPAP were significantly increased（P0.05）,the marked increase of wall thickness were observed in H group and the level of serum ROS in H group were significantly increased（P0.05）.Furthermore,the changes above were inhibited by NAC treatment. Conclusion Oxidative stress caused by hypoxia plays an important role in the development of hypoxic pulmonary hypertension.