中性粒细胞弹性蛋白酶在重症急性胰腺炎并急性肺损伤发病中的表达和意义

Expression and significance of neutrophil elastase in the pathogenesis of severe acute pancreatitis combined with acute lung injury

目的探讨中性粒细胞弹性蛋白酶(NE)在重症急性胰腺炎并急性肺损伤(SAP-ALI)发病过程中的作用和意义。方法健康SD大鼠60只,随机分成3组:假手术组(n=18)、SAP-ALI组(n=22)、NE处理组(n=20)。ELISA法测定血清NE、TNF-α、IL-1β含量,RT-PCR法检测肺组织中NE的变化,Western blot检测肺组织中NE的表达,ELISA法检测肺泡灌洗液(BALF)和血清中的NE的含量变化,同时观察肺和胰腺组织湿/干质量比和病理形态学变化。结果 NE处理组肺组织、BALF和血清NE含量均较假手术组和SAP-ALI组大鼠明显增加(P<0.05或P<0.01),血清TNF-α、IL-1β含量也明显增加(P<0.05)。结论中性粒细胞的大量激活及其NE、TNF-α、IL-1β的过度释放参与了SAP-ALI的发病过程。

Objective To investigate the expression and significance of neutrophil elastase（NE） in the pathogenesis of severe acute pancreatitis combined with acute lung injury（SAP-ALI）. Methods Sixty health SD rats were randomly divided into three groups： sham operation group（n=18）,SAP-ALI group（n=22）,NE group（n=20）.The TNF-α and IL-1β in blood was measured with ELISA method.The expression of NE in the lungs was detected by RT-PCR and Western blot,and the content of NE in BALF and serum was also detected by ELISA.The wet-to-dry（W/D） ratio of lung tissues,and pathomorphological changes in pancreatitis and lung tissue was observed. Results The contents of NE in lung tissue,BALF and serum of NE group were significantly higher than that of sham group and SAP-ALI group（P0.05 or P0.01）.The contents of TNF-α and IL-1β in NE group were also significantly higher than that of sham group and SAP-ALI group（P0.05）. Conclusion Many neutrophil activation and excessive release of NE,TNF-α and IL-1β were involved in the pathogenesis of SAP-ALI.