布渣叶药材质量标准的初步研究

Study on the initial quality standard of Microcos Paniculata L.

目的建立布渣叶的质量标准。方法采用TLC法对布渣叶药材进行定性鉴别,HPLC法测定布渣叶中牡荆苷和水仙苷的含量。结果建立了布渣叶的薄层鉴别方法。牡荆苷在0.1～4μg范围内线性关系良好(r=0.999 6),平均加样回收率为96.44%,RSD为1.68%;水仙苷在0.1～4μg范围内线性关系良好(r=0.999 6),平均加样回收率为97.16%,RSD为1.85%。结论建立的布渣叶薄层鉴别和有效成分含量控制方法简便、准确,可用于布渣叶的质量控制。

Objective To establish the quality standard of Microcos Paniculata L..Methods M.Paniculata was identified with TLC,and the contents of vitexin and narcissin were determined by HPLC.Results TLC identification for M.Paniculata was established.The calibration curve was linear in the range of 0.1-4 μg（r=0.999 6） for vitexin and the average recovery was 96.44% with RSD of 1.68%;The calibration curve was linear in the range of 0.1-4 μg（r=0.999 6） for narcissin and the average recovery of narcissin was 97.16% with RSD of 1.85%.Conclustion The method of TLC and assay of the active ingredients from M.Paniculata were convenient and accurate,which may be used in its quality control.