PEDF对氧化应激损伤的RGC-5细胞的保护作用

Neuroprotective effects of PEDF against oxidative stress-induced injury in RGC-5 cells

目的:研究色素上皮衍生因子(PEDF)对分化的视网膜神经节细胞(RGC-5)氧化应激损伤的防护作用。方法:利用外源性活性氧(H2O2)造成分化的RGC-5细胞的氧化应激损伤,同时应用PEDF进行保护。用MTT检测其细胞活性,用Annexin V-FITC凋亡试剂盒测定凋亡。结果:H2O2以浓度依赖的方式降低RGC-5细胞活性,200μmoL/LH2O2在24h可以导致分化的RGC-5细胞活性降低40%;细胞凋亡百分数(36.60%±2.12%)较空白对照组(6.03%±1.45%)明显增加(P<0.01)。100ng/mLPEDF使H2O2损伤分化后RGC-5细胞的细胞活性提高了30%,凋亡率降低为(9.36±1.61)%。结论:PEDF能够有效地防护氧化应激对视网膜神经节细胞的损伤,是一种极具开发潜力的神经保护性药物。

AIM：To investigate the neuroprotective effects of pigment epithelium-derived factor（PEDF） against oxidative stress-induced injury in differentiated retinal ganglion cell （RGC）-5 cells.METHODS：After differentiated with staurosporine, RGC-5 cells were divided into 4 groups. After every group cells were cultured for 24 hours, cell viability was determined by MTT assay; the apoptosis percent was tested by the Annexin V-FITC apoptosis kit. RESULTS：H2O2 may induce cell death of RGC-5 cells in a concentration-dependant manner. Exposure to 200μmol/L H2O2 clearly induced cell death in 40% of the cells within 24 hours. The apoptosis percent （36.60%±2.12%） after exposure to 200μmol/L H2O2 is higher than the control cells（6.03%±1.45%）. PEDF at 100ng/mL suppressed the cell death induced by H2O2 about 30% of the control cells. At the same concentration, PEDF also inhibited the cell apoptosis induced by H2O2（P0.01）.CONCLUSION：PEDF is able to effectively protect RGC against oxidative stressed injury and can be used as a very potential neuroprotective drug.