摘要
目的:研究色素上皮衍生因子(PEDF)对分化的视网膜神经节细胞(RGC-5)氧化应激损伤的防护作用。方法:利用外源性活性氧(H2O2)造成分化的RGC-5细胞的氧化应激损伤,同时应用PEDF进行保护。用MTT检测其细胞活性,用Annexin V-FITC凋亡试剂盒测定凋亡。结果:H2O2以浓度依赖的方式降低RGC-5细胞活性,200μmoL/LH2O2在24h可以导致分化的RGC-5细胞活性降低40%;细胞凋亡百分数(36.60%±2.12%)较空白对照组(6.03%±1.45%)明显增加(P<0.01)。100ng/mLPEDF使H2O2损伤分化后RGC-5细胞的细胞活性提高了30%,凋亡率降低为(9.36±1.61)%。结论:PEDF能够有效地防护氧化应激对视网膜神经节细胞的损伤,是一种极具开发潜力的神经保护性药物。
AIM:To investigate the neuroprotective effects of pigment epithelium-derived factor(PEDF) against oxidative stress-induced injury in differentiated retinal ganglion cell (RGC)-5 cells.METHODS:After differentiated with staurosporine, RGC-5 cells were divided into 4 groups. After every group cells were cultured for 24 hours, cell viability was determined by MTT assay; the apoptosis percent was tested by the Annexin V-FITC apoptosis kit. RESULTS:H2O2 may induce cell death of RGC-5 cells in a concentration-dependant manner. Exposure to 200μmol/L H2O2 clearly induced cell death in 40% of the cells within 24 hours. The apoptosis percent (36.60%±2.12%) after exposure to 200μmol/L H2O2 is higher than the control cells(6.03%±1.45%). PEDF at 100ng/mL suppressed the cell death induced by H2O2 about 30% of the control cells. At the same concentration, PEDF also inhibited the cell apoptosis induced by H2O2(P0.01).CONCLUSION:PEDF is able to effectively protect RGC against oxidative stressed injury and can be used as a very potential neuroprotective drug.
出处
《国际眼科杂志》
CAS
2012年第9期1633-1635,共3页
International Eye Science
基金
国家自然科学基金项目(No.81100665)
陕西省科技厅资助项目(No.2011K14-02-07)
陕西省卫生厅资助项目(No.2010D56)
陕西省教育厅资助项目(No.2010JK807)
西安医学院资助项目(No.10FC01)~~