电针“足三里”对失血性休克延迟补液大鼠肝组织缺血性损伤的影响

Effects of electroacupuncture at "Zusanli"(ST 36) on hepatic ischemic injury in rats with delayed fluid replacement after hemorrhagic shock

目的:研究电针"足三里"对失血性休克延迟补液大鼠肝组织血流量、含水率以及血浆谷丙转氨酶(ALT)的作用,为电针足三里治疗失血性休克提供依据。方法:取40只SD雄性大鼠按全血容量的40%放血制成失血性休克模型,再随机分为4组,模型对照组(NT),立即补液组(IFR),电针足三里延迟补液组(EA/DFR)和电针非经穴延迟补液组(SEA/DFR),每组10只。NT组造模后不做任何治疗处理;IFR组于失血后10min即行补液;EA/DFR组于失血后10min电针"足三里"穴,SEA/DFR组于失血后10min电针非经穴部位,其他操作同EA/DFR组,两组均失血后3h实施延迟补液。测定失血前及失血后3h、12h的肝组织血流量、ALT含量及失血后12h的肝组织含水率。结果:失血后IFR组和EA/DFR组各指标与NT组比较均有显著改善(均P<0.05),而SEA/DFR组与NT组间各指标无显著差异。失血后3h,IFR组的肝组织血流量显著高于NT组、EA/DFR组及SEA/DFR组(均P<0.05),血浆ALT显著低于NT组、EA/DFR组及SEA/DFR组(均P<0.05),EA/DFR组血浆ALT水平显著低于NT组、SEA/DFR组(均P<0.05),而肝组织血流量与SEA/DFR组比较无显著差别(P>0.05)。失血后12h,EA/DFR组和IFR组血浆ALT水平和肝组织含水率均显著低于NT组、SEA/DFR组(均P<0.05),肝组织血流量均显著高于NT组、SEA/DFR组(均P<0.05);而IFR组的血浆ALT显著低于EA/DFR组(P<0.05),肝组织血流量显著高于EA/DFR组(P<0.05)。结论:电针"足三里"穴对失血性休克延迟补液大鼠肝组织缺血性损伤具有一定保护作用。

Objective To investigate the effects of electroacupuncture at ＂Zusanli＂ （ST 36） on the volume of hepatic blood flow, water ratio and plasma alanine aminotransferase （ALT） in rats with delayed fluid replacement after hemorrhagic shock and to provide the references for electroacupuncture at Zusanli （ST 36） in treating hemor rhagic shock. Methods Forty SD rats with hemorrhagic shock induced by bloodletting 40% of whole hlood volume were randomly divided into a hemorrhage with no treatment （NT） group, an immediate fluid replacement （1FR） group, an electroacupuncture at Zusanli （ST 36） and delayed fluid resuscitation （EA/DFR） group and a sham dee- troacupuncture and delayed fluid replacement （SEA/DFR） group, 10 rats in each group. No treatment was per formed in NT group. IFR group was treated with fluid replacement at 10 minutes after blood loss, and EA/DFR group was treated with eleetroacupuncture at ＂Zusanli＂ （ST 36） at 10 minutes after blood loss, while non-acupoint was punctured in SEA/DFR group. Two EA groups were received delayed fluid replacement at 3 hours after blood loss. The volume of hepatic blood flow and ALT before blood loss and 3 h and 12 h after blood loss. and water ra- tio 12 h after blood loss were measured. Results After blood loss. all parameters in IFR group and EA/DFR group were improved significantly in contrast with those in NT group （all P〈0.05）. There was no significantdifference between SEA/DFR group and NT group. Three hours after blood loss, the hepatic blood flow of IFR group was significant higher than those of NT group, EA/DFR group and SEA/DFR group （all P〈0.05）, while the plasma AI.T of IFR group was significant lower than those of NT group, gA/DFR group and SEA/DFR group （all P〈0.05）, and the plasma ALT of EA/DFR group was lower than those of NT group and SEA/DFR group （botb P〈0.05）, the hepatic blood flow of EA/DFR group showed no significant difference compared with that of SEA/DFR group （P〉0.05）. Twelve hours after blood loss, the plasma ALT and the water ratio of EA/DFR group and IFR group were significant lower than those of NT group and SEA/DFR group （all P〈0.05）, and the hepatic blood flow of EA/DFR group and IFR group was significant higher than those of NT group and SEA/DFR group （all P〈0.05）, while the plasma ALT of IFR group was significant lower than that of EA/DFR group （P〈 0.05）, and the hepatic blood flow of IFR group was significant higher than that of EA/DFR group （P〈.0.05）. Conclusion Electroacupuncture at ＂Zusanli＂ （ST 36） has a protective effects for hepatic isehemic injury in rats with delayed fluid replacement afier hemorrhagic shock.