摘要
利用 RAPD技术 ,从 50 0个随机寡核苷酸引物 ( 10聚体 )中筛选出 8个引物能在 3个主栽的汕优系统杂交稻组合汕优 63、汕优 64和汕优晚 3及其亲本之间稳定地扩增出 12个强的多态性标记。利用这些多态性标记能够有效地区别汕优 63、汕优 64和汕优晚
A total of 500 arbitrary 10 mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) technique on the genome DNAs of Shanyou 63、 Shanyou 64、 Shanyouwan 3 and their parents.Eight primers produced twelve obviously repeatable polymorphic markers.Using these RAPD markers, the rice hybrid combinations (Shanyou 63、 Shanyou 64 and Shanyouwan 3) and their parents can be identified.
出处
《作物学报》
CAS
CSCD
北大核心
2000年第3期292-296,共5页
Acta Agronomica Sinica
基金
安徽省政府专项基金资助种子工程项目
关键词
RAPD技术
多态性标记
杂交水稻
鉴定
RAPD(random amplified polymorphic DNA)technique
Polymorphic markers
Hybrid combination of rice
Identification
