S100A9蛋白在胃癌中的表达及其临床病理意义

Expression and Clinicopathologic Significance of S100A9 Protein in Gastric Carcinoma

目的:探讨胃癌(GC)中S100A9的表达及临床病理意义。方法:蛋白质组织学定量鉴定GC和癌旁胃黏膜(GM)组织的差异表达蛋白质;Western-blot验证蛋白S100A9的表达;免疫组织化学检测S100A9蛋白在GC、GM和原发性胃癌转移的淋巴结(LN)的表达;分析S100A9表达与GC临床病理参数的关系。结果:蛋白质组织学筛选的78个差异蛋白中,S100A9表达在GC中较正常GM中明显上调(1∶0.09),Western-blot验证S100A9蛋白在GC表达上调(P<0.01);免疫组织化学显示:70例正常GM中S100A9阳性表达23例(32.86%);118例GC中S100A9阳性表达72例(61.02%);35例LN中S100A9阳性表达29例(82.86%),与正常GM相比,GC和LN中S100A9的表达明显升高(P<0.01);S100A9在LN的GC中上调(P<0.05);S100A9表达与GC的淋巴结转移、分化程度、浸润深度和TNM分期相关(P<0.01或P<0.05),而与患者的年龄和性别无关(P>0.05)。结论:S100A9高表达与GC发生发展、侵袭转移有关,可能是GC的一个促癌因子。

Objective： This study aims to investigate the expression and clinicopathologic significance of the S 100A9 protein in gastric cancer （ GC ）. Methods： Differential expression of proteins between GC and paraneoplastic gastric mucosa （ GM ） tissues was quantitatively determined using proteomics. The S100A9 protein level was examined using western blot analysis. The S 100A9 protein expression in GC, GM, and lymph node （ LN ） of primarily gastric carcinomatous metastasis was determined using immunohistochemistry （ IHC ）. The relationship between S100A9 protein expression and the clinicopathologic parameters of GC was analyzed. Results： Among the 78 differential proteins identified through proteomics, S 100A9 protein expression was clearly upregulated in GC compared with the GM （ 1：0.09 ）, which was confirmed through western blot analysis （ P 〈 0.01 ）. The IHC assay showed the positive expression of 23 S100A9 proteins （ 32.86 % ） among 70 normal GC samples, 72 （ 61.02 % ） among 118 GM samples, and 29 （ 82.86 % ） among 35 LN samples. Compared with GM, S100A9 expression was significantly increased in GC and LN （ P 〈 0.01 ）. S100A9 expression was upregulated in LN compared with GC （ P 〈 0.05 ）. The S100A9 expression level in GC was correlated with LN metastasis, histologic differentiation, depth of invasion, and TNM stage （ P 〈 0.01 or P 〈 0.05 ）, but not correlated with the patient age and gender （ P 〉 0.05 ）. Conclusion： S 100A9 protein overexpression is correlated with GC carcinogenesis, invasion, and metastasis and it may promote GC.