玫瑰茄提取物对Wistar大鼠肾Na^+-K^+-ATP酶及Ca^(2+)-Mg^(2+)-ATP酶活性的影响(英文)

Effects of aqueous extract of Hibiscus sabdariffa on renal Na^+-K^+-ATPase and Ca^(2+)-Mg^(2+)-ATPase activities in Wistar rats

目的:研究口服玫瑰茄水提取物对大鼠肾 Na+-K+-ATP酶和Ca2+ -Mg2+-ATP酶活性的影响。方法:连续28d分别给予实验大鼠口服25和50mg/kg的玫瑰茄水提物,同时给予对照组大鼠灌胃适当剂量的蒸馏水。用光谱测定法分析大鼠肾脏中 Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性。结果:口服25和50mg/kg的玫瑰茄提取物后,实验组大鼠肾Ca2+-Mg2+-ATP酶活性显著降低(P<0.05),然而肾 Na+-K+-ATP酶的活性却未受到任何影响。实验组大鼠体质量、肾脏质量,血浆白蛋白和总蛋白浓度,碱性磷酸酶、天冬氨酸氨基转移酶、丙氨酸氨基转移酶活性以及血浆钠、钾、钙离子的浓度与对照组大鼠相比无明显变化;但大鼠的肌酐以及尿素水平均在服用50mg/kg的玫瑰茄提取物后明显降低(P<0.05)。结论:尽管口服玫瑰茄提取物会引起肾 Ca2+-Mg2+-ATP酶活性的减弱,但同时可以起到保护肾脏功能的作用。

OBJECTIVE： To investigate the effects of oral administration of aqueous extract of Hibiscus sabdariffa on renal Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase activities in rats. METHODS： The 25 and 50 mg/（kg · d） of aqueous extracts of H. sabdariffa were respectively given to rats in the experimental groups for 28 d, and rats in the control group received an appropriate volume of distilled water as vehicle. Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase activities in the kidney were assayed by spectrophotometric method. RESULTS： Administrations of 25 and 50 mg/（kg · d） of aqueous extract of H. sabdariffa significantly decreased the Ca2＋-Mg2＋-ATPase activity in the kidney of rats （P 〈 0. 05）. However, the renal Na＋-K＋-ATPase activity of the experimental rats was not affected by either dose of the extract. And the plasma Na＋ , K＋ and Ca2＋ levels of the experimental rats had no significant changes. Administration of either dose of the extract did not result in any significant changes in body and kidney weights, the concentrations of plasma albumin and total protein, and alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase activities. However, concentrations of creatinine and urea were significantly reduced by 50 mg/kg of the extract （P〈0.05）. CONCLUSION： The present study indicates that oral administration of aqueous extract of H. sabdariffa may preserve the renal function despite a decreased renal Ca2＋-Mg2＋-ATPase activity.