西藏长叶松树皮乙醇提取物的抗惊厥作用(英文)

Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg.

目的:研究西藏长叶松树皮乙醇提取物(alcoholic extract of bark of Pinus roxburghii Sarg.,AEPR)的抗惊厥作用。方法:用最大电休克和戊四唑诱发白化 Wistar大鼠癫痫。最大电休克模型经由大鼠耳电极给予150mA 电流刺激0.2s诱发癫痫,以后肢强直性伸展持续时间的变化作为结局指标衡量 AEPR的抗惊厥作用,即后肢强直性伸展持续时间减少或停止。戊四唑模型大鼠经腹膜内注射戊四唑诱发肌阵挛性发作和阵发性抽搐,以痉挛发作的延迟衡量 AEPR的抗惊厥作用。结果:在最大电休克惊厥模型中,AEPR剂量分别为300和500mg/kg体质量,均显著减轻所有阶段的大鼠惊厥发作(P<0.01);标准对照药物苯妥英钠组用量25mg/kg,能显著减轻屈曲阶段的发作(P<0.01),并抑制所有阶段的惊厥发作。戊四唑惊厥模型大鼠于注射戊四唑前30min分别给予 AEPR 300和500mg/kg,均能显著延迟阵挛性发作(P<0.01)。100mg/kg体质量的 AEPR在戊四唑诱发的癫痫模型中没有显著的抗惊厥作用;标准对照药物地西泮4mg/kg能大幅度延迟阵挛性发作。结论:本研究提示 AEPR能有效抑制普遍强直性肌阵挛和部分癫痫发作。因此,AEPR对最大电休克及戊四唑诱发的大鼠癫痫有抗惊厥作用。然而需要进一步的研究确认是 AEPR中的何种成分对这种抗惊厥作用起主导作用。

OBJECTIVE： To study the anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. （AEPR） used in Indian traditional medicine system in treating convulsion. METHODS： Anticonvulsant activity was evaluated by maximal electroshock （MES） and penty- lenetetrazole （PTZ）-induced seizures in Wistar albino rats. In the MES model, 150 mA current for 0.2 s was given through ear electrodes to induce convulsions in rats. The duration of tonic extension of hind limb was used as the end point, namely, prevention or decrease in the duration of hind limb extension was considered as a protective action. In the PTZ model, the anticonvulsant property of AEPR was assessed by its ability to delay the onset of myoclonic spasm and clonic convulsions produced by intraperitoneal administration of PTZ. RESULTS： In the MES-induced seizure model, AEPR in doses of 300 and 500 mg/kg body weight reduced all the phases of convulsion significantly （P〈0. 0]）. Standard drug phenytoin at a dose of 25 mg/kg significantly reduced flexion phase （P〈0.01） and abolished all phases of convulsion. In the PTZ-induced seizure model, the administration of the extract at doses of 300 and 500 mg/kg 30 min prior to injection of PTZ significantly delayed the onset of clonic seizure （P〈0.01）. AEPR at the dose of 100 mg/kg body weight could not exert any significant protec- tive effect on PTZ-induced convulsions. Standard drug diazepam at a dose of 4： mg/kg showed much delayed onset of clonic seizure. CONCLUSION： The study suggests that AEPR would be effective against generalized tonicclonic and partial seizures. Thus AEPR possesses anticonvulsant property against MES- and PTZ-induced seizures in Wistar rats. However, further research is in progress to isolate the compound responsible for its activity.