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以流感病毒RNA聚合酶为靶点的抗病毒药物筛选和药效学评价方法的建立 被引量：5

Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase
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摘要 流感病毒RNA聚合酶对流感病毒基因组的复制和表达至关重要。编码聚合酶各亚基的基因序列高度保守的特点使其成为极具发展潜力的抗流感药物靶标。本研究通过构建流感病毒RNA聚合酶活性依赖的荧光素酶报告系统,在细胞水平上建立了以流感病毒RNA聚合酶为靶点的抗流感药物筛选模型。特异性评价和统计学分析表明,该筛选方法灵敏可靠、重复性好,可以用于针对流感病毒RNA聚合酶的抗流感药物筛选。 Influenza virus RNA-dependent RNA polymerase （RdRP） is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
作者 王臻 王歆 贺贞 刘振龙 魏晓露 殷霄 周金明 李晓宇 张志珍 岑山
机构地区 中国医学科学院、北京协和医学院医药生物技术研究所免疫生物学室 广东医学院生物化学与分子生物学教研室
出处 《药学学报》 CAS CSCD 北大核心 2012年第9期1159-1163,共5页 Acta Pharmaceutica Sinica
关键词 流感病毒 抗流感药物 筛选模型 RNA聚合酶 报告系统 influenza virus anti-influenza drug screening assay RNA polymerase reporter system
分类号 R965.1 [医药卫生—药理学]
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参考文献13

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同被引文献88

  • 1杨益良,梁国栋,付士红,何海怀,李晓宇,邓娟,苏乃伦,王力华,侯云德.中国首次分离的辛德毕斯病毒XJ-160病毒株感染性全基因组cDNA克隆的构建与分析[J].病毒学报,2005,21(3):173-180. 被引量：10
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  • 9Bearman G M, Shankaran S, Elam K. Treatment of severe cases of pandemic (H1N1) 2009 influenza; review of anti- virals and adjuvant therapy [J]. Recent Pat Antiinfect Drug Discov, 2010, 5(2):152-156.
  • 10Abed Y, Goyette N, Boivin G. Generation and characteriza- tion of recombinant influenza A (H1N1) viruses harboring amantadine resistance mutations [J]. Antimicrob Agents Chemother, 2005, 49: 556-559.

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药学学报
2012年 第9期

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