电化学发光法检测神经元特异性烯醇化酶影响因素的分析

The analysis of influencing factors for neuron-specific enolase detected by electrochemiluminescence

目的:探讨血液标本放置不同时间后分离血清对神经元特异性烯醇化酶(NSE)的影响。方法:采用电化学发光法分别对25例健康检验者的全血标本在室温(25℃)放置30min、1h、2h、3h后再分离血清,并设两组溶血样品。将1h组血清取出部分放置-20℃冰冻3天作为冰冻组,各组血清进行NSE水平测定。结果:1h组、2h组与30min组比较无统计学差异(P>0.05),3h组与30min组、溶血组与1h组比较,结果差异有统计学意义(P<0.01)。结论:血液标本放置2h以上对NSE测定有一定影响,冰冻血清存放复融后对结果无影响,而溶血对NSE测定结果有明显影响。

Objective：To study the clinical changes of content of neuron specific enolase （NSE） in patient serum within different time. Methods： Serum NSE contents were measured with electrochemiluminescence within 30min, 1 h,2h,3h in 25 healthy people and in two controls （hemolysis and frozen serum）. Results： Serum contents of NSE on healthy people within 1 h, 2h were not significantly different from healthy people within 30min （ P 〉 0.05 ） ; But ser- um contents of NSE within 3h were significantly higher than those within 30min （P 〈 0.01 ） , the same to hemolysis and serum contents of NSE within lh. Conclusion： Serum NSE levels beyond 2h were not significantly increased,but hemolysis were significantly increased, however, frozen serum has no influence to serum NSE levels.