垂体腺瘤侵袭性与质地的相关性研究

Correlation between invasion and texture in human pituitary adenomas

目的探讨垂体腺瘤侵袭性与质地的关系。方法收集2008年1月～2010年1月经病理证实的垂体腺瘤患者76例,其中功能腺瘤44例,无功能腺瘤32例;根据术前影像学检查分为侵袭组32例,非侵袭组44例;根据术中判断分为质地软组54例和质地硬组22例。应用免疫组化SP法检测76例垂体腺瘤标本中Ki-67和基质金属蛋白酶2(MMP2)的表达水平。统计分析垂体腺瘤侵袭性与Ki-67和MMP2表达的关系及垂体腺瘤侵袭性与质地的关系。结果侵袭组Ki-67和MMP2的表达水平分别为7.573±3.991和18.623±9.689,显著高于非侵袭组2.811±2.095和6.871±7.230(t1=6.161,t2=5.788,P<0.01),Ki-67与MMP2表达呈正相关(r=0.476,P<0.01)。垂体腺瘤的侵袭性与质地无明显相关性(P=0.056)。结论 Ki-67和MMP2可作为诊断垂体腺瘤侵袭性的分子标记物,检测Ki-67和MMP2表达水平可指导临床评价垂体腺瘤侵袭性及制定个体化治疗方案,而术前仅依靠垂体腺瘤的侵袭性影像学特征并不能判断其质地。

Objective To detect the correlation between the invasiveness and texture of pituitary adenomas （PA）. Methods 76 cases of PA being verified surgically and pathologically were collected at our hospital from January 2008 to January 2010, then were divided into different groups based on different criteria： invasive group （32 cases ） and non-invasive group （44 eases ） according to their MRI finding; hard group （22 cases ） and soft group （54 eases ） in texture according to surgical finding. Immunohistoehemieal staining （SP method ） was used for detecting the expression of Ki-67 and MMP2 （matrix metalloproteinase2 ） in these samples of PA. The correlation between the invasiveness and the expressions of Ki-67 and MMP2, and the correlation between the invasiveness and the texture of PA was statistically analyzed . Results The expression of Ki - 6 7 and MMP 2 in invasive group was 7. 573 ± 3. 991 and 18. 623 ± 9. 689 respectively, which significantly higher than the one in non - invasive group （2.811 ±2.095 and 6.871±7.230, tl =6. 161, t2 =5.788, P 〈0.01）. Ki-67 was positively correlated with MMP2 （ r = 0. 476, P 〈 0.01 ）. There was no correlation being found between the invasiveness and the texture of PA （ P = 0. 056 ）. Conclusion Ki-67 and MMP2 can be used as molecular markers to evaluate the invasiveness of PA, therefore to guide the individual therapy. The texture of PA can not be determined simply by its radiologieal features of invasiveness.