EphA2与VEGF蛋白在喉鳞癌组织中的表达及相关性研究

The expression and correlation of EphA2 and VEGF protein in laryngeal squamous cell carcinoma

目的探讨沉默促红细胞生成素产生肝细胞受体(erythropoietin-producing hepatocellular re-ceptor,EphA2)与促血管生成因子(VEGF)蛋白在喉鳞状细胞癌(喉鳞癌)组织中的表达及相关性。方法采用免疫组织化学技术检测EphA2、VEGF蛋白在69例喉鳞癌组织及15例癌旁黏膜组织中的表达,分析二者表达水平与临床病理特征的关系。结果 EphA2和VEGF蛋白在喉鳞癌组织中的阳性率均高于癌旁黏膜组织(P均<0.05),EphA2蛋白表达尚与肿瘤原发部位相关(P<0.05),且EphA2和VEGF蛋白表达均与喉鳞癌患者的临床分期(P均<0.05)及转移(P均<0.05)密切相关,而二者表达与患者年龄、性别、组织学分级均无明显相关(P均>0.05)。喉鳞癌组织中EphA2及VEGF蛋白表达存在正相关性(P<0.05)。生存分析结果显示EphA2及VEGF蛋白表达水平与患者5年总生存率密切相关(P<0.05)。多因素Cox比例风险回归模型进一步显示,淋巴结有无转移及EphA2蛋白表达水平为喉鳞癌患者预后的独立影响因素。结论 EphA2与VEGF蛋白在喉鳞癌组织中表达均上调,提示二者可能存在相互作用,共同在喉鳞癌的发生、发展中起作用。

Objective To investigate the expression and biological significance of EphA2 and VEGF protein in tissue specimens of laryngeal squamous cell carcinoma （ LSCC ） , and further study the correlation between the protein expression of EphA2 and VEGF. Methods The expression of EphA2 and VEGF protein was evaluated by immunohistochemieal staining in 69 cases of LSCC specimens and 15 cases of adjcent epithelial tissue samples, and their correlation with clinicopathologic parameters was analyzed. Results The positive rates of EphA2 and VEGF in LSCC tissues were significantly higher than those in adjacent non-cancerous mucosa （ P 〈 0. 05 ） , and the expression of these two marks was closely correlated with clinical stage （ P 〈 0.05 ） and metastasis （ P 〈 0.05 ） in patients with LSCC. Furthermore, the expression of EphA2 was also associated with tumor site （ P 〈 0. 05 ）. However, the expression of EphA2 and VEGF had no significant association with age, sex and histological differentiation （ P 〉 0 . 0 5 ） . There was a positive correlation between the expression of EphA 2 and VEGF （ P 〈 0.05 ）. Kaplan-Meier survival analysis demonstrated that patients with high expression of EphA2 or VEGF had poorer overall survival compared with that in patients with relative low EphA2 or VEGF expression （P 〈 0.,05 ）. Multivariate COX regression analysis revealed that both lymph node metastasis and EphA2 expression were independent prognostic factors for patients with LSCC. Conclusion Increased protein expression of EphA2 and VEGF in LSCC suggested that EphA2 and VEGF might play a critical role in the initiation and progression of LSCC.