摘要
The inhibitory effects of methanol extracts from the tissues of three macroalgal species on the growths of three marine red tide microalgae were assessed under laboratory conditions.Extracts of Sargassum thunbergii(Mertens ex Roth) Kuntz tissue had stronger inhibitory effects than those of either Sargassum pallidum(Turner) C.Agardh or Sargassum kjellmanianum Yendo on the growths of Heterosigma akashiwo(Hada) Hada,Skeletonema costatum(Grev.) Grev,and Prorocentrum micans Ehrenberg.Methanol extracts of S.thunbergii were further divided into petroleum ether,ethyl acetate,butanol,and distilled water phases by liquid-liquid fractionation.The petroleum ether and ethyl acetate fractions had strong algicidal effects on the microalgae.Gas chromatography-mass spectrometry analyses of these two phases identified nine fatty acids,most of which were unsaturated fatty acids.In addition,pure compounds of four of the nine unsaturated fatty acids had effective concentrations below 5 mg/L.Therefore,unsaturated fatty acids are a component of the allelochemicals in S.thunbergii tissue.
The inhibitory effects of methanol extracts from the tissues of three macroalgal species on the growths of three marine red tide microalgae were assessed under laboratory conditions. Extracts of Sargassum thunbergii （Mertens ex Roth） Kuntz tissue had stronger inhibitory effects than those of either Sargassumpallidum （Turner） C. Agardh or Sargassum kjellmanianum Yendo on the growths of Heterosigma akashiwo （Hada） Hada, Skeletonema costatum （Grev.） Grev, and Prorocentrum micans Ehrenberg. Methanol extracts ofS. thunbergii were further divided into petroleum ether, ethyl acetate, butanol, and distilled water phases by liquid-liquid fractionation. The petroleum ether and ethyl acetate fractions had strong algicidal effects on the microalgae. Gas chromatography-mass spectrometry analyses of these two phases identified nine fatty acids, most of which were unsaturated fatty acids. In addition, pure compounds of four of the nine unsaturated fatty acids had effective concentrations below 5 mg/L. Therefore, unsaturated fatty acids are a component of the allelochemicals in S. thunbergii tissue.
基金
Supported by the National Natural Science Foundation of China(No. 31070458)
the National Key Technology R&D Program of China(No. 2010BAC68B00)
the Specialized Research Program for Marine Public Welfare Industry from the State Oceanic Administration of China(Nos. 200805066,200905020,201005007)
the Natural Science Foundation of Shandong Province(No.2009ZRB01461)
the Encouraging Foundation for Outstanding Youth Scientists of China(No.2008BS09011)
the Science and Technology Project of Institutions of Higher Education of Shandong(No.J10LC13)
关键词
有毒化合物
海洋赤潮
鼠尾藻
微藻
不饱和脂肪酸
甲醇提取物
鉴定
乙酸乙酯
allelopathic effects
Heterosigma akashiwo
Skeletonema costatum
Prorocentrum micans
unsaturated fatty acids
gas chromatography-mass spectrometry （GC-MS）
