蚊虫连续石蜡切片制备及染色

Preparation and staining of serial paraffin slices of mosquitoes.

本研究利用塑化石蜡进行了吸血前后白纹伊蚊整个蚊虫的石蜡包埋、制片及 HE染色 ,获得了染色清晰 ,组织完整的连续石蜡切片 ,并讨论了有关蚊虫石蜡包埋及制片染色的一些注意事项。为进一步利用免疫组化、原位

Whole Aedes albopictus was embeded in paraplast before and after meal blood. Serial paraffin sections were made and HE staining was processed. Tissues of the whole mosquito exhibited clear and different staining. This technique is valuable in studying arbovirus location or distribution in mosquitoes when combined with immunocytochemical or in situ PCR and hybridyzation assays.