期刊文献+

质粒DNA制备方法的改进 被引量:1

下载PDF
导出
摘要 目的 :改进碱裂解制备质粒DNA的方法。方法 :在小规模制备中 ,采用 7 5mol/LNH4 Ac中和碱溶液及室温下异丙醇沉淀 ;在中规模制备中 ,采用三步沉淀将质粒分离纯化 ;在大规模制备中 ,用两步清洗弃去由于量大而不宜除掉的杂物 (碎屑物、杂蛋白等 )。结果 :建立了一套快速、高效、无毒、经济的方法。
出处 《生物学杂志》 CAS CSCD 2000年第3期35-35,共1页 Journal of Biology
  • 相关文献

同被引文献9

  • 1O'Reilly MS, Holmgren L,Shing Y,et al. Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastasis by Lewis lung carcinoma[J]. Cell,1994,79(2) :315-328.
  • 2Cao Y, Ji RW, Davidson D,et al. Kringle domains of human angiostatin [J]. J Biol Chem, 1996,271:29461-29467.
  • 3Boehn T,Forkman J,Browder T et al. Antongiogenic therapy of experiment cancer dose not induce acquired drug resistance [J ]. Nature, 1997,390 : 404- 407.
  • 4Soff GA. Angiostatin and angiostatin-related proteins [J]. Cancer Metastasis Rev, 2000,19(1-2) :97- 107.
  • 5Chha Vakis E,Dimm eler S. Regulation of endothelial cell survival and apoptosis during angiogenesis [J]. Arterioscler Throwb Vasc Biol, 2002,22 (16 ) : 887 - 893.
  • 6Folkman Judah. Role of angiogenesis in tumor growth and metastasis [J]. Semin Oncol,2002,29(6 suppl 16):15-18.
  • 7O'Reilly MS, Holmgren L,Shing Y,et al. Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastasis by Lewis lung carcinoma [J]. Cell,1994,79(2) :315-328.
  • 8Boehn T,Forkman J,Browder T et al. Antongiogenic therapy of experiment cancer dose not induce acquired drug resistance [J]. Nature, 1997,390 : 404- 407.
  • 9Soff GA, Angiostatin and angiostatin-related proteins [J], Cancer Metastasis Rev, 2000,19(1-2) :97- 107.

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部