小鼠睾丸表皮生长因子受体及增殖细胞核抗原表达的加龄变化研究

Study on the Age-Specific Changes of EGFR and PCNA in the Seminiferous Epithelium of the Mouse

观察了表皮生长因子受体及增殖细胞核抗原在生后 1天至生后 1 0月龄昆明种小鼠睾丸内的表达 ,结果表明 :精原细胞及初级精母细胞从生后第 2周至生后 4周龄DNA复制旺盛 ,增殖细胞核抗原免疫反应阳性细胞面密度于生后 1 4天出现峰值。表皮生长因子受体在间质细胞、精母细胞内均有表达。生后 4周时 ,精母细胞表皮生长因子受体表达较强 ,便于表皮生长因子发挥调节细胞增殖、凋亡的作用。

The express of EGFR and PCNA in the seminiferous epithelium was observed during a series of developing stages from birth to postnatal 10th month with SP method. The results show that: between 2nd and 4th weeks after birth replicating activity of DNA in spermatogonia and primary spermatocytes were flourishing. Surface densities of PCNA\|positive cells reached up their peaks on 14th day after birth through measurements of IP\|8 computer image analysis system. There were expression of EGFR in Leydig cells and spermatocytes, and the stronger expression of EGFR was located at spermatocytes during postnatal fourth week.