猕猴自然感染肝片吸虫诱发胆管结石的形成

A Cholesterol-Bilirubinate Gallstone Induced by Fasciola hepatica Infection in Macaca mulatta

在研究肝片吸虫诱发猕猴自发性胆结石形成的病理学基础上 ,采用红外光谱分析、原子吸收光谱分析和组织化学染色对胆结石的成分及结构进行了测定 ,初步探讨了本病发生的机理。在一只 9岁雌性猕猴肝总胆管内发现 4条肝片吸虫 (Fasciolahepatica) ,胆囊胆汁中检出大量肝片吸虫虫卵。左侧胆管内有一颗棕黑色结石 ,直径为 1cm、长约 2 5cm圆柱形。肝细胞灶性坏死伴有轻度结缔组织增生 ,胆管腺体重度增生 ,上皮细胞胞质内含有中性与酸性混合型粘多糖物质 ,上皮间有大量杯状细胞。胆石切面呈环状 ,环层间含有粘多糖物质。胆石经红外光谱分析出现胆固醇 -胆色素混合型胆石和黑色物质特征性吸收峰 ;

The present paper showed one case of cholesterol\|bilirubinate gallstone in 9 years old female Rhesus Monkey.This monkey was infected by 4 pieces of Fasciola hepatica naturally in the ductus communis and a black brown gallstone was formed in ductus hepaticus sinister.The liver cells was necrotic locally and the epithelia cell of bile ductules were deeply grown with a lot of AB/PAS positive materials in the cytoplasm. The infrared spectroscopy analysis of the gallstone showed it had characteric absorption bands of cholesterol bilirubinate gallstone and a lot of black materal, Q =0.11. The results of atomic absorption spectroscopy analysis showed the four metal contents in the gallstone as follow: Ca 36 6ppm,Fe 9 1ppm,Mg 3 4ppm,and K 10 9ppm. A lot of substances with the positive to PAS and AB stainning could be found outside the lane of gallstone.