抑癌基因maspin在肝癌组织中的表达及其临床意义

Expression of Tumor Suppressor Gene Maspin in Hepatocellular Carcinoma and Its Clinical Significance

目的探讨抑癌基因maspin在肝癌组织中的表达及其临床意义。方法选取肝癌组织及其癌旁肝组织各32例,另选择正常肝脏组织30例作为对照,采用免疫组化法检测3种组织中maspin基因的表达情况,并分析maspin基因表达与肝癌临床参数的关系。结果肝癌组织maspin基因阳性率(28.1%)显著低于癌旁组织(62.5%)和正常肝组织(86.7%);肝癌组织中maspin基因表达阳性率与肿瘤分化程度、有无肝内外转移有关(P<0.05),与年龄、性别、肿瘤直径、TMN分期和有无门静脉侵犯无关(P>0.05)。结论 maspin基因在肝癌组织中呈低表达,其表达情况可作为判断肝癌进展情况和恶性程度的指标之一。

Objective To explore the expression of turmor suppressor gene maspin in hepatocellular carcinoma and its clinical significance. Methods Thirty two patients with hepatocellular carcinoma were enrolled in the study,and 30 health cases as controls. Immunohistochemistry was used to detect the expressions of maspin in the targeted hepatocellular carcinoma tissue ,adjacent surrounding liver tissue and normal liver tissue,and relationship between maspin expression and clinical pathological parameters of hepatocellular carcinaoma was analyzed. Results The positive rate of maspin in the targeted hepatocellular carcinoma tissue （28.1% ） was significantly lower than that in the adjacent surrounding liver tissue （62.5 % ） as well as normal liver tissue （86.7 % ）. The positive rate of maspin expression was related with tumor differentiation and metastasis （P 〈 0.05 ）, but was not related with age, gender, the diameter of tumor, TMN stage and portal vein invasion（P 〉0.05 ）. Conclusion Maspin shows a low expression in targeted hepatocellular carcinoma tissue, and its expression can be used as an index for the judgment of development and severity of bepatocellular carcinoma.