炙山茱萸高效液相色谱指纹图谱研究

HPLC fingerprint of Corni Fructus Praeparata Cum Melle

目的建立炙山茱萸高效液相色谱(HPLC)指纹图谱。方法色谱柱为Dikma Diamonsil C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-水(含0.08%磷酸)为流动相梯度洗脱;柱温:40℃;流速:0.8 ml/min(0～50 min),0.8～1.0 ml/min(50～60 min);检测波长:276 nm(0～15 min),236 nm(15～46 min),276 nm(46～60 min);进样量:20μl。采用Q-TOF-MS-IDA-MS/MS方法对22个共有峰中的16个色谱峰进行了定性鉴别。采用该方法测定了10批不同来源的炙山茱萸。结果该方法精密度、稳定性和重复性良好。结论该研究为炙山茱萸的全面质量评价奠定了基础。

Objective To establish the fingerprint of Corni Fructus Praeparata Cum Melle by HPLC.Methods The chromatographic fingerprint was obtained with Dikma Diamonsil C18 column（250 mm × 4.6 mm,5 μm） and a gradient elution with the mobile phase consisted of acetonitrile and 0.08% phosphoric water.The column temperature was maintained at 40 ℃,and the flow rate was 0.8 ml/min（0-50 min）,0.8-1.0 ml/min（50-60min）.The detection wavelength was 276 nm（0-15 min）,236 nm（15-46 min） and 276 nm（46-60 min）.The injection volume was 20 μl.Sixteen chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method.Ten batches of Corni Fructus Praeparata Cum Melle were determined.Results Good precision,stability and repeatability were obtained.Conclusion The studies lay a foundation for a comprehensive quality evaluation of Corni Fructus Praeparata Cum Melle.