快速起搏大鼠原代培养心房肌细胞内皮素-1表达的变化(英文)

Expressions of endothelin-1in primary cultured atrial myocytes during early rapid pacing

目的:摘要:目的 :利用原代培养的心房肌细胞建立快速起搏模型,研究在快速起搏早期内皮素-1(ET-1)表达的变化。方法:以原代培养大鼠心房肌细胞,建立快速起搏细胞模型,利用酶联免疫吸附(ELISA)法和逆转录聚合酶链式反应(RT-PCR)方法检测在快速起搏3、6、12、24 h后内皮素-1的mRNA表达和分泌量的变化。结果:与起搏前比较,快速起搏6 h后ET-1的mRNA表达[(0.31±0.02)比(0.52±0.07)、(0.62±0.09)、(0.75±0.05)]和分泌量[(3.4±0.8)ng/L比(6.0±1.4)ng/L、(8.3±1.5)ng/L、(11.2±2.1)ng/L]持续增加(P均<0.01)。结论:快速起搏早期,原代培养心房肌细胞内皮素-1的mRNA表达和分泌量随起搏时间增加,提示内皮素-1参与了房颤时心房病理重构。

Objective ： To study the expressions of endothelin-1 in primary cultured atrial myocyte model at early stages of atrial fibrillation by rapid pacing.Methods ： The primary rat atrial myocytes were cultured,in which a rapid paced cell model was established.The polymerase chain reaction（RT-PCR） and enzyme-linked immunosorbent assay（ELISA） were applied to detect the messenger ribonucleic acid（mRNA） expression and secretion of endothelin1 on 3h,6h,12h and24h after rapid pacing.Results ： Compared with before pacing,there were continuous and significant increase in expression of ET-1mRNA [（0.31±0.02） vs.（0.52±0.07）,（0.62±0.09）,（0.75±0.05）] and secretion of endothelin-1 [（3.4±0.8） ng / L vs.（6.0±1.4） ng / L,（8.3±1.5） ng / L,（11.2±2.1） ng / L ] on 6h,12h and 24h after rapid pacing,P0.01 all.Conclusion ： Expressions of ET-1mRNA and secretion of endothelin-1 significantly increase depend on time in early rapid pacing atrial myocytes,indicating that endothelin-1 participates in atrial pathological remodeling during atrial fibrillation.