潍坊、花园口土样中土著大豆根瘤菌的遗传多样性研究

GENETIC DIVERSITY OF INDIGENOUS SOYBEAN RHIZOBIA ISOLATED FROM SOILS IN WEIFANG AND HUAYUANKOU

采用 16S 2 3SrDNA间隔区段 (IGS)RFLP分析和RAPD分析技术 ,分别对分离自山东潍坊和河南郑州花园口的 2 4株土著大豆根瘤菌进行了遗传多样性分析 .结果表明 ,在 70 %的相似性水平上 ,依IGS RFLP分析可将供试菌株分为 10群 ,依RAPD分析可将供试菌株分为 8群 .综合两种分析技术在 5 0 %的相似性水平上将供试菌株分为潍坊群和花园口群 .表明其在系统发育和分子进化上有着明显的地理分隔作用 .图 6表 2参

S 23S rDNA intergenic spacer(IGS) RFLP and randomly amplified polymorphic DNA(RAPD) were used to analyze the genetic diversity of 24 indigents soybean rhizobial strains isolated from soils in Weifang and Huayuankou. The results showed that all strains tested could be divided into 10 or 8 small clusters at the similarity level of 70% by 16S 23S rDNA IGS RFLP and RAPD, respectively. According the combined results of IGS RFLP and RAPD, all strains tested could be divided into Weifang and Huayuankou clusters at the similarity level of 50% or so, which showed geographical insulation in systematic development and molecular evolution. The difference of results between IGS RFLP and RAPD demonstrated that total DNA and the IGS sequence had heterogeneous characteristic and IGS RFLP was more sensitive for detecting genetic diversity of soybean rhizobia. Fig 6 Tab 2 Ref 8