固定CO_2氢细菌的筛选及其培养条件优化

SCREENING AND CULTURE CONDITIONS OF HYDROGEN OXIDIZING BACTERIA

从土壤中分离筛选到一株高效固定CO2 的氢氧化细菌—专性化能自养菌BHA 15 ,在最佳培养条件下(培养基 ρ/gL-1:NH4 Cl 4.0 ,Na2 HPO4 ·12H2 O 0 .75 ,KH2 PO4 0 .5 ,MgSO4 ·7H2 O 0 .3;V(H2 )∶V(O2 )∶V(CO2 ) =3 .5∶1∶1;pH =7;最适生长温度θ =30℃ ) ,密闭摇瓶中恒温 (30℃ )培养 40h后 ,菌体浓度 ρ(DW)达 1.6mg/mL ,菌体蛋白含量 71.8% .图 7表 2参

An obligately chemolithoautotrophic bacterium using CO 2 as sole carbon source for growth was isolated from soil. The optimum growth pH and temperature were 7.0 and 30℃, respectively. Ammonium or nitrate nitrogen was well utilized for growth. At the optimum growth conditions(g L -1 :NH 4CL 4.0, Na 2HPO 4·12H 2O 0.75, KH 2PO 4 0.5, MgSO 4·7H 2O 0.3;H 2∶O 2∶CO 2=3.5∶1∶1; pH=7), 1.6 mg cell dry weight/ml broth was obtained with 718 mg protein/g cell dry weight within 40 hours in a close culture system. Fig 7, Tab 2, Ref 12