基因克隆的常用方法介绍

A Brief Introduction to the Methods for Novel Gene Cloning

为能快速、准确地克隆出有意义的基因,本文介绍了目前常用的一些基因克隆方法,如差异显示PCR、抑制性差减杂交、RAPPCR、代表性差异显示、酵母双杂交系统、cDNA直接捕捉法等;并对这些方法作了简要的评价,以利于大家选择适合自己的方法。

There are a lot of methods for novel gene cloning, but how to clone candidate gene(s) quickly and correctly? This is a brief introduction to methods of novel gene cloning, these methods includes: differential display reverse transcriptase polymerase chain reaction(DD RT PCR),suppression subtractive hybridization(SSH),RNA arbitrarily primed PCR(RAP PCR), representational difference analysis(RDA), yeast two hybrid system, cDNA capturation, et al. We not only introduced these methods, but also discussed the advantages and disadvantages of them. However, no single method is omnipotent, one should pick up the method most suitable for a special purpose.