苏云金芽胞杆菌YBT-1520杀虫晶体蛋白基因的属性

CHARACTERIZATION OF THE INSECTICIDAL CRYSTAL PROTEIN GENES OF BACILLUS THURINGIENSIS YBT-1520

通过Southern杂交发现高毒力苏云金芽胞杆菌 (Bacillusthuringiensis)YBT 1 52 0菌株含有两个杀虫晶体蛋白基因片段 ,其 5’ 末端所在HindⅢ片段分别为 6 8kb和 4 6kb ,它们对应的基因分别命名为cry2 1 8和cry4 6。经PCR鉴定 ,该菌含有cry1Aa、cry1Ab和cry1Ac基因 ,以及cry2基因 ,其中cry2 1 8属于cry1Ac。分析了cry2 1 8基因 41 90bp的核苷酸序列 ,在杀虫晶体蛋白基因分类系统中被命名为cry1Ac1 0。结合Southern杂交和PCR结果可判断 3个cry1A基因的拷贝数不同 ,其中cry1Ac拷贝数最高 ,YBT 1 52 0菌株与其它库斯塔克亚种的杀虫晶体蛋白基因所在限制性内切酶位置明显不同。

The insecticidal crystal protein genes of highly toxic \%Bacillus thuringiensis\% YBT\|1520 were analyzed. Southern hybridization and PCR analysis indicated that this strain bears \%cry1Aa, cry1Ab,cry1Ac\% and \%cry2\% genes. The copy number of three \%cry1A\% genes is different and \%cry1Ac\% gene (here named \%cry218\%) is the highest. The restriction location of three genes is different from that in typical strains, such as HD 1 and HD 73 strains. 4190bp nucleotide sequence of \%cry218\% gene was determined and this gene was named \%cry1Ac10\% in the recently published gene nomenclature.