红曲菌产生的DPPH自由基捕捉物质的筛选

SCREENING DPPH RADICAL SCAVENGERS FROM MONASCUS SP.

从浙江长兴县农村传统家酿红酒的小曲中分离出一红曲菌菌株 (Monascussp .) ,对它产生的DPPH自由基捕捉物质进行了筛选。结果表明 :自由基捕捉活性和得率以醋酸乙酯的中性提取物较高 ;培养时间以 30℃ ,1 0 0r/min摇床 5d为好 ;对醋酸乙酯的中性提取物进一步进行了硅胶柱层析、LH 2 0柱层析、MPLC、HPLC分部收集 ,并用HPLC检验其纯度 ,获得收量在 2mg以上 ,纯度在 85%以上的自由基捕捉物质 1 5个 ,对其中有代表性的B1 3、C3 1 7进行了1 H NMR和1 3C NMR分析 ,确定它们的大致结构为苯的三取代衍生物 ,C3 1 7很可能是 3 羟基 ,4 甲氧基苯甲酸 ;并对B1 3、C3 1 7的自由基捕捉活性进行了测定 ,40 μmol/L的B1 3其自由基捕捉活性约为 65% ,40 μmol/L的C3 1 7其自由基捕捉活性小于 56% ,均略低于Vc和Ve。

A strain belongs to Monascus sp. was separated from hongqu(Chinese beni coji) which was used to brew red wine in Changxin county, Zhejiang province, and its DPPH radical scavenging metabolites were studied. Results showed that the yield and radical scavenging activity of neutral extract with EtOAc were higher than that of acid and basic extract. After shaken at 30℃, 100r/min for 5 days, this strain produced largest amount of neutral extract. Further partitioning the neutral fraction with silica gel column chromatograph, LH 20 column chromatograph, MPLC and HPLC, we screened 15 free radical scavengers whose yield was more than 2 mg and purity was higher than 85% in HPLC(260 nm absorption). Several of them were analyzed with 1H NMR and 13 C NMR spectrum from which we concluded that B1 3 and C3 1 7 were two kinds of substitute of benzene, and C3 1 7 may be 3 OH 4 OCH 3 benzoic acid. The scavenging activity of 40μmol/L B1 3 was about 65%, and that of C3 1 7 was lower than 56%, while as the control the scavenging activity of 40μmol/L Vc and Ve was 80.4% and 78.4% respectively.