摘要
Using pulsed\|field gel electrophoresis (PFGE),two indigenous large plasmids were isolated from Streptomyces T8 4.Two dimentional PFGE revealed that both plasmids were linear molecules.By parallel electrophoresis with linear plasmids of known sizes,the two linear plasmids were estimated to be approximately 230kb and 90kb,which were designated as pHZ1000 and pHZ1001,respectively.Both plasmids could be transferred into S.lividans ZX1 by conjugation,which is detectable by “pock”formation.Five S.lividans ZX1 derivatives which carry one or two plasmids were isolated and characterized by Southern hybridization and PFGE.
Using pulsed\|field gel electrophoresis (PFGE),two indigenous large plasmids were isolated from Streptomyces T8 4.Two dimentional PFGE revealed that both plasmids were linear molecules.By parallel electrophoresis with linear plasmids of known sizes,the two linear plasmids were estimated to be approximately 230kb and 90kb,which were designated as pHZ1000 and pHZ1001,respectively.Both plasmids could be transferred into S.lividans ZX1 by conjugation,which is detectable by “pock”formation.Five S.lividans ZX1 derivatives which carry one or two plasmids were isolated and characterized by Southern hybridization and PFGE.
出处
《微生物学报》
CAS
CSCD
北大核心
2000年第4期435-439,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金!资助项目 ( 3 9770 3 96和 3 983 0 2 1 0 )
