虫草素改善脑缺血小鼠学习记忆及对海马神经元数量的影响

Promotion of Behavior-Learning and Effect on Number of Hippocampal Neurons of Mice Injured by Ischemia with Cordycepin Treatment

采用双侧颈动脉夹闭建立脑缺血模型,观察造模后和造模前腹腔注射100 mg/kg虫草素对小鼠Y迷宫行为训练的影响,以及检测海马各区神经元数量的变化.结果表明,造模前给予虫草素能明显提高小鼠的正确反应率(P<0.05),减少达标所需训练次数(P<0.05),显著增加海马CA1区和CA3区锥体神经元数量(P<0.01).造模后给予虫草素显著减少小鼠达标所需训练次数(P<0.05);同时,海马CA3区神经元数量显著增加(P<0.01).由此可见,虫草素能改善脑缺血小鼠的学习能力,预防作用比治疗作用更为显著,可能与虫草素促进海马神经元的修复有关.

To investigate effects of cordycepin on learning we processed ischemic mice model by 2 - vessel occlusion, and hippocampal neurons of mice injured by ischemia, and observed Y maze training of mice with post - or pre - treatment of 100 mg/kg cordycepin （ i. p. ）, which was followed by the detection of hippocampal neurons density in CA1 , CA3 and DG region. The results indicated that pre - treatment of cordycepin increased rates of correct re- sponses （RCR） and decreased trails of reaching standard （TRS） significantly （ P 〈0. 05 ）, with an increased num- ber of hippocampal pyramidal neurons both in CA1 and CA3 region （P 〈 0. 01 ）. On the other hand, post - treat- ment of cordycepin remarkably reduced TRS （P 〈 0. 05 ） without distinguished changes of RCR （P 〉 0. 05） and only improved the number of pyramidal neurons in hippocampal CA3 region （P 〈 0. 01 ）. Together, we concluded that cordycepin conspicuously ameliorated behavioral learning of mice injured by ischemia, which was more effec- tive with pre - treatment.