应用RAPD技术研究阿尔卑斯山黄花茅居群内的遗传分化(英文)

Subpopulation differentiation of Anthoxanthum alpinum (Poaceae)along an altitudinal gradient detected by random amplifiedpolymor phic DNA

应用RAPD技术 ,沿一个海拔梯度研究了阿尔卑斯山黄花茅自然居群的遗传变异和分化。实验表明 ,虽然在亚居群间有很少的亚居群独有遗传标记的存在 ,但通过RAPD资料的聚类分析、主因子分析以及相关分析观察到遗传分化沿海拔梯度发生 ,而且亚居群间的遗传分化和它们的海拔高度 (地理距离 )呈有意义的正相关。研究结果暗示 ,在阿尔卑斯山的高山 亚高山过渡区 ,亚居群间的海拔高度差别可能导致黄花茅开花和生长物候期的变化 ,而后者限制了亚居群间的基因流 ,从而引起居群内的遗传分化。

RandomamplifiedpolymorphicDNA (RAPD)phenotypesgeneratedby 1 5primerswere scoredin 5subpopulationsofAnthoxanthumalpinumalonganaltitudinalgradient.Althoughfew subpopulation specificmarkerswerefound ,asignificantpopulationdifferentiationwasrevealedby theuseofprincipalfactoranalysisandWard’sclusteranalysis.Theresultwasalsoconfirmedby thesignificantpositivecorrelationwhencomparingboththematrixofsimilaritybetweentheindividu alswithinsubpopulationsandthematrixamongsubpopulations,aswellasthematrixofdistances andthedifferencesofaltitudebetweenthesubpopulations (Manteltest) .Thisstudysuggeststhat thedifferencesofaltitudebetweensubpopulationsitesmayresultinthedifferencesofphenological periodforfloweringandgrowthwhichrestrictgeneflow .